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@thesis{thesis,
author={Millenia Maria},
title ={Deteksi escherichia coli dan total bakteri pada feses cicak rumah},
year={2022},
url={http://erepository.uwks.ac.id/12240/},
abstract={ABSTRAK
Penelitian ini dilakukan untuk mengetahui keberadaan bakteri Escherichia coli
serta mengetahui total bakteri yang berada pada feses cicak rumah. Sejumlah 30
ekor cicak rumah diambil dari penjual cicak di Surabaya dengan kriteria SVL 4-
6cm dan tinggal di kawasan indoor perumahan. Sampel cicak dibawa menuju
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Wijaya
Kusuma Surabaya untuk diperiksa. Sampel yang digunakan ialah feses cicak segar
sejumlah 0,1 gram. Pemeriksaan bakteri Escherichia coli dilakukan dengan
penanaman bakteri pada media agar EMBA dengan metode streak, pemeriksaan
mikroskop dengan perbesaran 1000x, dilanjutkan dengan Uji Biokimia indol,
methyl red, voges proskauer, sitrat (IMViC) serta Triple Sugar Iron Agar (TSIA).
Sedangkan pemeriksaan Total Plate Count feses cicak dilakukan dengan
penanaman bakteri menggunakan Nutrient agar pada pengenceran 105
dan 106
dengan metode tuang kemudian dihitung secara manual dan diolah hasil datanya
menggunakan rumus TPC. Hasil penelitian menunjukan bahwa 5 dari 30 cicak
rumah positif bakteri Escherichia coli dan memiliki total rata-rata TPC sebesar
2x108 CFU/ml.
Kata kunci: Escherichia coli, cicak rumah, feses, total plate count
ABSTRACT
This research was conducted to find out the existence of Escherichia coli and total
bacteria in house gecko?s feces. 30 house geckos were taken from house gecko
seller in Surabaya with requirements of SVL 4 to 6cm and live indoor. House gecko
samples brought to Microbiology Laboratorium of Wijaya Kusuma Surabaya
University to be examined. 0,1gram of fresh house gecko feces were used as
samples. Escherichia coli detection was conducted with bacteria isolation in EMB
agar using streak method, microscope examination with 1000x magnification, and
biochemical test such as indole, methyl red, voges proskauer, citrate (IMViC), and
Triple Sugar Iron Agar (TSIA). While Total Plate Count of house gecko feces were
conducted with bacteria with dilution of 105
and 106 isolated in nutrient agar using
pour plate method. The results were counted manually and processed with TPC
formula. Research result shows that 5 out of 30 house geckos have Escherichia coli
bacteria and average of Total plate count are 2x108 CFU/ml.
Keywords: Escherichia coli, house gecko, feses, total plate count}
}