EFEK ANTIOKSIDAN EKSTRAK DAUN RAMBUSA (Passiflora foetida) DAN
TAURIN TERHADAP RESPON HISTOPATOLOGI HATI MENCIT YANG
DIINDUKSI PARAQUAT
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Institusion
Universitas Lampung
Author
RETNO KHUSNIATI ROFIQOH, 1317021062
Subject
QH301 Biology
Datestamp
2017-04-27 02:10:25
Abstract :
ABSTRAK Paraquat merupakan senyawa toksik yang dapat menginduksi terbentuknya molekul ROS (ReactiveOxygen Species). ROS yang terbentuk akibat paparan paraquat dapat menurunkan aktivitas antioksidan tubuh, sehingga menyebabkan kerusakan pada berbagai organ vital seperti hati. Untuk mengurangi kerusakan yang ditimbulkan akibat aktivitas ROS, diperlukan sumber antioksidan seperti taurin yang mampu menangkap radikal bebas dan menghentikan produksi molekul ROS yang dihasilkan akibat paparan paraquat. Selain taurine, daun Rambusa (Passiflora foetida) memiliki kandungan senyawa polifenol dan flavonoid yang berperan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui kemampuan daun rambusa dan taurin dalam memperbaiki histopatologi sel hepar mencit yang mengalami kerusakan. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) yang terbagi dalam 4 kelompok perlakuan dengan masing-masing 6 ulangan Kelompok K0 (diberi pakan standard dan air minum hingga akhir penelitian), kelompok K1 (diinduksi praquat secara intraperitoneal dengan dosis 20 mg/kgBB sebanyak 6 kali selama 21 hari), kelompok K2 (diinduksi paraquat dengan dosis 20 mg/kgBB secara intraperitoneal sebanyak 6 kali selama 21 hari dan rambusa dengan dosis 500mg/kgBB/hari selama 21 hari), kelompok K3 (diinduksi paraquat dengan dosis 20 mg/kgBB secara intraperitoneal sebanyak 6 kali selama 21 hari + rambusa dengan dosis 500mg/kgBB/hari selama 21 hari, dan taurin dengan dosis 15,6 mg/mencit/hari selama 21 hari). Hasil analisa dengan uji one way Anova (p<0.05) dilanjutkan dengan uji Beda Nyata Terkecil (BNT), menunjukan pemberian kombinasi ekstrak daun rambusa dan taurine efektif dalam melindungi jaringan hepar mencit jantan akibat induksi paraquat. Kata kunci : Histopatologi hepar, Paraquat,Rambusa ROS,Taurin. ABSTRACT Paraquat is a toxic compound wich induce the molecules of ROS (Reactive Oxygen Species) through a series of cellular processes. ROS formed by paraquat can reduce the activity of antioxidants in the body, causing damage to vital organs such as the liver. To reduce the damage of ROS?s activity, needed source of antioxidants such as Taurine which able to capture free radicals and stop the production of ROS molecules from paraquat exposure. In addition to taurine, Passiflora foetida leaves are also a source of antioxidants. Passiflora foetida leaves contain polifenol and flavonoids which act as antioxidants.This study aims to determine the effect of Passiflora foetida leaves dan Taurine on the liver to protect histopathological of mice which suffe from ROS damage. This study was conducted in a complete randomized design by using four treatments (K0, K1, K2 and K3), each in six replications. K0 used as control were not given any treatment. K2 were induced by 20 mg/bw paraquat intraperitonialy six time for 21 days, for 10 days, K2 were given 20 mg/bw paraquat intraperitonialy six time for 21 days, and 500 mg/bw of rambusa leaves extract for 21 days. K3 were given 20 mg/bw paraquat intraperitonialy six time for 21 days, given 15,6 mg/bw of taurine which was combined by 500 mg/bw of rambusa leaves extract. The data were analyzed using ANOVA (Analysis of Variance) then continued by calculating least significant difference at 0,05 level of significance. The results indicated that taurine which was combined by rambusa leaves ethanolic extract had the ability to protect hepar form the damage caused by paraquat herbiside. Key words : Liver histopatology, paraquat, Passiflora foetida, ROS, Taurine.
ABSTRAK Paraquat merupakan senyawa toksik yang dapat menginduksi terbentuknya molekul ROS (ReactiveOxygen Species). ROS yang terbentuk akibat paparan paraquat dapat menurunkan aktivitas antioksidan tubuh, sehingga menyebabkan kerusakan pada berbagai organ vital seperti hati. Untuk mengurangi kerusakan yang ditimbulkan akibat aktivitas ROS, diperlukan sumber antioksidan seperti taurin yang mampu menangkap radikal bebas dan menghentikan produksi molekul ROS yang dihasilkan akibat paparan paraquat. Selain taurine, daun Rambusa (Passiflora foetida) memiliki kandungan senyawa polifenol dan flavonoid yang berperan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui kemampuan daun rambusa dan taurin dalam memperbaiki histopatologi sel hepar mencit yang mengalami kerusakan. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) yang terbagi dalam 4 kelompok perlakuan dengan masing-masing 6 ulangan Kelompok K0 (diberi pakan standard dan air minum hingga akhir penelitian), kelompok K1 (diinduksi praquat secara intraperitoneal dengan dosis 20 mg/kgBB sebanyak 6 kali selama 21 hari), kelompok K2 (diinduksi paraquat dengan dosis 20 mg/kgBB secara intraperitoneal sebanyak 6 kali selama 21 hari dan rambusa dengan dosis 500mg/kgBB/hari selama 21 hari), kelompok K3 (diinduksi paraquat dengan dosis 20 mg/kgBB secara intraperitoneal sebanyak 6 kali selama 21 hari + rambusa dengan dosis 500mg/kgBB/hari selama 21 hari, dan taurin dengan dosis 15,6 mg/mencit/hari selama 21 hari). Hasil analisa dengan uji one way Anova (p<0.05) dilanjutkan dengan uji Beda Nyata Terkecil (BNT), menunjukan pemberian kombinasi ekstrak daun rambusa dan taurine efektif dalam melindungi jaringan hepar mencit jantan akibat induksi paraquat. Kata kunci : Histopatologi hepar, Paraquat,Rambusa ROS,Taurin. ABSTRACT Paraquat is a toxic compound wich induce the molecules of ROS (Reactive Oxygen Species) through a series of cellular processes. ROS formed by paraquat can reduce the activity of antioxidants in the body, causing damage to vital organs such as the liver. To reduce the damage of ROS?s activity, needed source of antioxidants such as Taurine which able to capture free radicals and stop the production of ROS molecules from paraquat exposure. In addition to taurine, Passiflora foetida leaves are also a source of antioxidants. Passiflora foetida leaves contain polifenol and flavonoids which act as antioxidants.This study aims to determine the effect of Passiflora foetida leaves dan Taurine on the liver to protect histopathological of mice which suffe from ROS damage. This study was conducted in a complete randomized design by using four treatments (K0, K1, K2 and K3), each in six replications. K0 used as control were not given any treatment. K2 were induced by 20 mg/bw paraquat intraperitonialy six time for 21 days, for 10 days, K2 were given 20 mg/bw paraquat intraperitonialy six time for 21 days, and 500 mg/bw of rambusa leaves extract for 21 days. K3 were given 20 mg/bw paraquat intraperitonialy six time for 21 days, given 15,6 mg/bw of taurine which was combined by 500 mg/bw of rambusa leaves extract. The data were analyzed using ANOVA (Analysis of Variance) then continued by calculating least significant difference at 0,05 level of significance. The results indicated that taurine which was combined by rambusa leaves ethanolic extract had the ability to protect hepar form the damage caused by paraquat herbiside. Key words : Liver histopatology, paraquat, Passiflora foetida, ROS, Taurine.
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