UJI DAYA HAMBAT LENDIR IKAN SIDAT, Anguilla bicolor (McClelland,
1844) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli
(INHIBITION POWER OF EEL FISH MUCUS, Anguilla Bicolor
(McClelland, 1844) TO Escherichia coli BACTERIA’S GROWTH)
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Institusion
Universitas Lampung
Author
Annisa Abdillah, 1418011022
Subject
RS Pharmacy and materia medica
Datestamp
2018-01-30 05:08:27
Abstract :
Background. In recent years, antibiotics drug found a resistance for many kind of bacteria, such as Escherichia coli. Because of it, the urgency to find a new drug that have sensitivity to Escherichia coli is needed. Eel fish of Anguilla bicolor have a potential antimicrobial in its mucus composition for future antibiotic drug. This research is aimed to know inhibition power of eel fish mucus, Anguilla bicolor (McClelland, 1844) to Escherichia coli bacteria?s growth. Methods. Present study was an experimental in vitro test with well diffusion method to show inhibition zone of eel fish mucus to Escherichia coli bacteria?s growth. This research has 24 trial unit with 3 repetition for 8 trial, that are eel fish mucus, Anguilla bicolor with concentration 100%, 50%, 25%, 12,5%, 6,25%, NaCl as control negative, ampicillin and chloramphenichol as control positive. Result. The result of this present study showed that the eel mucus from Anguilla bicolor (McClelland, 1844) has 0 mm inhibition zone of each consentration to Escherichia coli bacteria?s growth. Conclusion. Anguilla bicolor (McClelland, 1844) mucus has not antibacterial activity against Escherichia coli bacteria. Keyword: Anguilla bicolor, diameter of inhibition zone, eel fish mucus, Escherichia coli,. ABSTRAK Latar Belakang. Antibiotik telah mengalami berbagai resistensi, contohnya resistensi yang terjadi pada bakteri Escherichia coli. Resistensi antibiotik ini menyebabkan meningkatnya kebutuhan agen antibiotik baru yang masih sensitif terhadap Escherichia coli. Lendir ikan sidat memiliki potensi sebagai antibiotik. Penelitian ini bertujuan untuk mengetahui daya hambat lendir ikan sidat, Anguilla bicolor (McClelland, 1844) terhadap pertumbuhan bakteri Escherichia coli. Metode Penelitian. Penelitian ini dilakukan secara in vitro dengan menggunakan difusi sumuran untuk melihat daya hambat lendir ikan sidat terhadap pertumbuhan bakteri Escherichia coli. Pada penelitian ini terdapat 24 satuan percobaan dengan 3 kali ulangan untuk 8 perlakuan, yaitu lendir ikan sidat, Anguilla bicolor dengan konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, kontrol negatif NaCl, kontrol positif dengan menggunakan ampisilin dan kloramfenikol. Hasil Penelitian. Penelitian ini membuktikan bahwa lendir ikan sidat, Anguilla bicolor (McClelland, 1844) memiliki 0 mm daya hambat terhadap pertumbuhan bakteri Escherichia coli. Simpulan Penelitian. Ikan sidat, Anguilla bicolor (McClelland, 1844) tidak memiliki aktivitas antibakterial terhadap pertumbuhan bakteri Escherichia coli. Kata Kunci : Anguilla bicolor, diameter zona hambat, Escherichia coli, lendir ikan sidat.
Background. In recent years, antibiotics drug found a resistance for many kind of bacteria, such as Escherichia coli. Because of it, the urgency to find a new drug that have sensitivity to Escherichia coli is needed. Eel fish of Anguilla bicolor have a potential antimicrobial in its mucus composition for future antibiotic drug. This research is aimed to know inhibition power of eel fish mucus, Anguilla bicolor (McClelland, 1844) to Escherichia coli bacteria?s growth. Methods. Present study was an experimental in vitro test with well diffusion method to show inhibition zone of eel fish mucus to Escherichia coli bacteria?s growth. This research has 24 trial unit with 3 repetition for 8 trial, that are eel fish mucus, Anguilla bicolor with concentration 100%, 50%, 25%, 12,5%, 6,25%, NaCl as control negative, ampicillin and chloramphenichol as control positive. Result. The result of this present study showed that the eel mucus from Anguilla bicolor (McClelland, 1844) has 0 mm inhibition zone of each consentration to Escherichia coli bacteria?s growth. Conclusion. Anguilla bicolor (McClelland, 1844) mucus has not antibacterial activity against Escherichia coli bacteria. Keyword: Anguilla bicolor, diameter of inhibition zone, eel fish mucus, Escherichia coli,. ABSTRAK Latar Belakang. Antibiotik telah mengalami berbagai resistensi, contohnya resistensi yang terjadi pada bakteri Escherichia coli. Resistensi antibiotik ini menyebabkan meningkatnya kebutuhan agen antibiotik baru yang masih sensitif terhadap Escherichia coli. Lendir ikan sidat memiliki potensi sebagai antibiotik. Penelitian ini bertujuan untuk mengetahui daya hambat lendir ikan sidat, Anguilla bicolor (McClelland, 1844) terhadap pertumbuhan bakteri Escherichia coli. Metode Penelitian. Penelitian ini dilakukan secara in vitro dengan menggunakan difusi sumuran untuk melihat daya hambat lendir ikan sidat terhadap pertumbuhan bakteri Escherichia coli. Pada penelitian ini terdapat 24 satuan percobaan dengan 3 kali ulangan untuk 8 perlakuan, yaitu lendir ikan sidat, Anguilla bicolor dengan konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, kontrol negatif NaCl, kontrol positif dengan menggunakan ampisilin dan kloramfenikol. Hasil Penelitian. Penelitian ini membuktikan bahwa lendir ikan sidat, Anguilla bicolor (McClelland, 1844) memiliki 0 mm daya hambat terhadap pertumbuhan bakteri Escherichia coli. Simpulan Penelitian. Ikan sidat, Anguilla bicolor (McClelland, 1844) tidak memiliki aktivitas antibakterial terhadap pertumbuhan bakteri Escherichia coli. Kata Kunci : Anguilla bicolor, diameter zona hambat, Escherichia coli, lendir ikan sidat.
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