Perbedaan Jumlah Eritrosit Yang Dihomogenisasi Sekunder Manual Teknik Inversi 10 Kali Dengan Otomatis Teknik Rolling 1 Menit Dan 2 Menit
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Institusion
Universitas Katolik Musi Charitas
Author
Simbolon, Sarah Romian
Subject
RZ Other systems of medicine
Datestamp
2021-09-09 05:57:39
Abstract :
One of the hematological examinations is the examination of the number of erythrocytes. Factors that affect the number of erythrocytes are homogenization. There are 2 kinds of homogenization; manual homogenization and automatic homogenization. Objective : To find out the difference in the results of the examination of the number of erythrocytes which were homogenized by the inversion technique 10 times with automatic 1 minute and 2 minutes. Methods : This research is pre-experimental with static group comparison research design. The research subjects used were 32 TLM students. Blood samples were collected or collected in 3 tubes of EDTA anticoagulant, then the samples were primary homogenized 5 times and left for 5 minutes. Each blood sample was then subjected to manual secondary homogenization inversion 10 times, automatic homogenization 1 minute and 2 minutes. Results : 10 times manual inversion of homogenization technique have mean value of erythrocytes results; 4,73x106/µl, 1 minute automatic rolling technique homogenization have mean value of erythrocytes results; 4,82x106/µl, and 2-minutes automatic rolling technique homogenization has a mean value of erythrocytes results; 4,82x106/µl. The data in the normality test was normally distributed, then continued with repeated ANOVA test with the p value of manual 10 times inversion homogenization to 1 minute automatic homogenization; 0.039<0.05 which means there is a difference, p value of manual 10 times inversion homogenization for 2 minutes automatic homogenization; 0.029<0.05 which also means there is a difference, and the p value of 1 minute automatic homogenization to 2 minutes automatic homogenization; 1,000>0.05 which means there is no difference. Conclusion : There are differences in the results of the examination of the number of erythrocytes which are homogenized by the inversion technique 10 times with automatic 1 minute and 2 minutes and there is no difference in the results of the examination of the number of erythrocytes which are homogenized automatically for 1 minute and 2 minutes. Suggestion : For the automatic method, it is suggested to speed up the homogenization time to 30 seconds.
One of the hematological examinations is the examination of the number of erythrocytes. Factors that affect the number of erythrocytes are homogenization. There are 2 kinds of homogenization; manual homogenization and automatic homogenization. Objective : To find out the difference in the results of the examination of the number of erythrocytes which were homogenized by the inversion technique 10 times with automatic 1 minute and 2 minutes. Methods : This research is pre-experimental with static group comparison research design. The research subjects used were 32 TLM students. Blood samples were collected or collected in 3 tubes of EDTA anticoagulant, then the samples were primary homogenized 5 times and left for 5 minutes. Each blood sample was then subjected to manual secondary homogenization inversion 10 times, automatic homogenization 1 minute and 2 minutes. Results : 10 times manual inversion of homogenization technique have mean value of erythrocytes results; 4,73x106/µl, 1 minute automatic rolling technique homogenization have mean value of erythrocytes results; 4,82x106/µl, and 2-minutes automatic rolling technique homogenization has a mean value of erythrocytes results; 4,82x106/µl. The data in the normality test was normally distributed, then continued with repeated ANOVA test with the p value of manual 10 times inversion homogenization to 1 minute automatic homogenization; 0.039<0.05 which means there is a difference, p value of manual 10 times inversion homogenization for 2 minutes automatic homogenization; 0.029<0.05 which also means there is a difference, and the p value of 1 minute automatic homogenization to 2 minutes automatic homogenization; 1,000>0.05 which means there is no difference. Conclusion : There are differences in the results of the examination of the number of erythrocytes which are homogenized by the inversion technique 10 times with automatic 1 minute and 2 minutes and there is no difference in the results of the examination of the number of erythrocytes which are homogenized automatically for 1 minute and 2 minutes. Suggestion : For the automatic method, it is suggested to speed up the homogenization time to 30 seconds.
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TLM-2021-1734010-cover.pdf
TLM-2021-1734010-abstract.pdf
TLM-2021-1734010-complete.pdf
TLM-2021-1734010-summary_id.pdf
TLM-2021-1734010-tableofcontent.pdf
TLM-2021-1734010-chapter 1.pdf
TLM-2021-1734010-chapter 2.pdf
TLM-2021-1734010-chapter 3.pdf
TLM-2021-1734010-chapter 4.pdf
TLM-2021-1734010-conclusion.pdf
TLM-2021-1734010-bibliography.pdf
TLM-2021-1734010-attachment.pdf
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