Uji Efektivitas Ekstrak Metanol Daun Mangrove (Rhizophora apiculata) Terhadap Mortalitas Larva Aedes aegypti
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Institusion
Universitas Muhammadiyah Surakarta
Author
Inayah, Virjinia Mugi
, dr. Rochmadina Suci Bestari, M.Sc
, dr. Rochmadina Suci Bestari, M.Sc
Subject
1 Medicine (General)
Datestamp
2025-02-26 01:52:29
Abstract :
Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus and transmitted through the bite of Aedes mosquitoes, especially Aedes aegypti. The prevalence of dengue fever continues to increase dramatically in the world. Based on the epidemiological triangle, there are several risk factors that cause dengue fever, namely host factors, infectious agents and the environment. The government has carried out interventions to prevent and control dengue since 1970, but these efforts have not been able to slow down the rate of this disease nationally. Prevention and control of vectors in the form of the Aedes aegypti mosquito can be done using natural larvicides. The use of mangrove leaf extract (Rhizophora sp.) to control mosquito larvae has the potential to be a biolarvicide. Mangrove leaves contain larvicidal secondary metabolites which include flavonoids, alkoloids, tannins, saponins and terpenoids. This research was to determine the effectiveness of 70% methanol from mangrove leaves (Rhizophora apiculata) on the mortality of Aedes aegypti larvae. This research is a laboratory experiment with a post-test only controlled group design. The samples used were Aedes aegypti larvae instar II-IV. The research used extract concentrations of 0.5%, 1% and 2% with 5 repetitions and larval deaths were counted every 6 hours for 24 hours. Data normality and homogeneity tests used Shapiro-Wilk analysis with a p value <0.05. So a non-parametric test was carried out using Kruskal-Wallis and continued with the Mann-Whitney post hoc test. There is a significant difference in the Mann-Whitney test data for 24 hours. In the probit test of methanol extract of mangrove leaves, LC50 was 1.008% and LT50 was 15,389 hours.
Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus and transmitted through the bite of Aedes mosquitoes, especially Aedes aegypti. The prevalence of dengue fever continues to increase dramatically in the world. Based on the epidemiological triangle, there are several risk factors that cause dengue fever, namely host factors, infectious agents and the environment. The government has carried out interventions to prevent and control dengue since 1970, but these efforts have not been able to slow down the rate of this disease nationally. Prevention and control of vectors in the form of the Aedes aegypti mosquito can be done using natural larvicides. The use of mangrove leaf extract (Rhizophora sp.) to control mosquito larvae has the potential to be a biolarvicide. Mangrove leaves contain larvicidal secondary metabolites which include flavonoids, alkoloids, tannins, saponins and terpenoids. This research was to determine the effectiveness of 70% methanol from mangrove leaves (Rhizophora apiculata) on the mortality of Aedes aegypti larvae. This research is a laboratory experiment with a post-test only controlled group design. The samples used were Aedes aegypti larvae instar II-IV. The research used extract concentrations of 0.5%, 1% and 2% with 5 repetitions and larval deaths were counted every 6 hours for 24 hours. Data normality and homogeneity tests used Shapiro-Wilk analysis with a p value <0.05. So a non-parametric test was carried out using Kruskal-Wallis and continued with the Mann-Whitney post hoc test. There is a significant difference in the Mann-Whitney test data for 24 hours. In the probit test of methanol extract of mangrove leaves, LC50 was 1.008% and LT50 was 15,389 hours.
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