PENETAPAN KADAR GLUKOSA PADA BUNGA BROKOLI
(Brassica oleracea var Italica) MENGGUNAKAN METODE
SPEKTROFOTOMETRI UV-VIS
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Risdwita, Anvia Haristantya
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2020-12-23 06:21:35
Abstract :
Broccoli (Brassica oleracea var. italica) classified as green vegetables that contains carbohydrates especially low calorie glucoseso useful as a source of energy and can be used as a low carbohydrate diet menu.Parts of broccoli can be consumed is green flower head arranged tightly. The purpose of this research is to know the glucose levels in broccoli flower. Fresh broccoli flowers is mashed by blender. The extracts obtained are prepared with the addition of calcium carbonate, lead acetate and sodium oxalate. Results of the preparation were used for qualitative analysis with molisch test, fehling test, barfoed test and quantitative analysis by UV-Vis spectrophotometry at maximum wavelength of 627.0 nm with the addition of 0.1% anthrone reagent. Qualitative analysis of carbohydrates showed that the broccoli flower positive contains glucose. The results showed the average glucose levels in broccoli flowers was 674,39 mg/100g b /b with % KV of 1,6718%. Glucose levels in broccoli flowers are equivalent to 606,951 mg/100g of carbohydrates.
Broccoli (Brassica oleracea var. italica) classified as green vegetables that contains carbohydrates especially low calorie glucoseso useful as a source of energy and can be used as a low carbohydrate diet menu.Parts of broccoli can be consumed is green flower head arranged tightly. The purpose of this research is to know the glucose levels in broccoli flower. Fresh broccoli flowers is mashed by blender. The extracts obtained are prepared with the addition of calcium carbonate, lead acetate and sodium oxalate. Results of the preparation were used for qualitative analysis with molisch test, fehling test, barfoed test and quantitative analysis by UV-Vis spectrophotometry at maximum wavelength of 627.0 nm with the addition of 0.1% anthrone reagent. Qualitative analysis of carbohydrates showed that the broccoli flower positive contains glucose. The results showed the average glucose levels in broccoli flowers was 674,39 mg/100g b /b with % KV of 1,6718%. Glucose levels in broccoli flowers are equivalent to 606,951 mg/100g of carbohydrates.
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