Potensi Aktivitas Antibakteri Fungi Endofit batang parang romang (Boehmeria Virgata (G.Forst.) Guill) Terhadap Bakteri penyebab infeksi mulut dengan metode KLT-AutoBioautografi
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Institusion
Universitas Muslim Indonesia
Author
Rusli, Rusli
Subject
R Medicine (General)
Datestamp
2025-08-20 04:58:21
Abstract :
ABSTRACT ANDI RAFIQAH AULIA. Potential Antibacterial Activity of Endophytic Fungi of Parang Romang Stem (Boehmeria virgata (G. Forst.) Guill) Against Bacteria Causing Oral Infection With TLC-Bioautography Method (Suprvised by Rachmat Kosman and Rusli). Parang romang plant (Boehmeria virgata (G.Forst.) Guill) is known to have antibacterial activity. This study aims to determine the antibacterial activity of endophytic fungal isolates from parang romang stems against bacteria that cause oral infections using the TLC-Bioautography method. The research began with the isolation of endophytic fungi and antagonistic tests, then obtained 2 active isolates against the test bacteria, namely IFBPR-8 and IFBPR-9. Both isolates were then fermented for 14 days at room temperature using MYB (Maltose Yeast Broth) medium. The fermentation results were extracted using ethyl acetate to produce fermentate extract. The extract obtained was followed by TLC testing using cloroform:methanol (15:1) eluent. The TLC-Bioautography test of IFBPR-8 isolate obtained Rf values of 0,78 and 0,34, and IFBPR-9 isolate obtained Rf values of 0,78 and 0,29 which showed antibacterial activity against bacteria Aggregatibacter actinomycetencomitans, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus mutas. The identification of chemical components of endophytic fungal isolates from parang romang stems is positive for alkaloids and saponins. Key words: Antibacterial, Boehmeria virgata (G.Forst.) Guill, TLCBioautography
ABSTRACT ANDI RAFIQAH AULIA. Potential Antibacterial Activity of Endophytic Fungi of Parang Romang Stem (Boehmeria virgata (G. Forst.) Guill) Against Bacteria Causing Oral Infection With TLC-Bioautography Method (Suprvised by Rachmat Kosman and Rusli). Parang romang plant (Boehmeria virgata (G.Forst.) Guill) is known to have antibacterial activity. This study aims to determine the antibacterial activity of endophytic fungal isolates from parang romang stems against bacteria that cause oral infections using the TLC-Bioautography method. The research began with the isolation of endophytic fungi and antagonistic tests, then obtained 2 active isolates against the test bacteria, namely IFBPR-8 and IFBPR-9. Both isolates were then fermented for 14 days at room temperature using MYB (Maltose Yeast Broth) medium. The fermentation results were extracted using ethyl acetate to produce fermentate extract. The extract obtained was followed by TLC testing using cloroform:methanol (15:1) eluent. The TLC-Bioautography test of IFBPR-8 isolate obtained Rf values of 0,78 and 0,34, and IFBPR-9 isolate obtained Rf values of 0,78 and 0,29 which showed antibacterial activity against bacteria Aggregatibacter actinomycetencomitans, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus mutas. The identification of chemical components of endophytic fungal isolates from parang romang stems is positive for alkaloids and saponins. Key words: Antibacterial, Boehmeria virgata (G.Forst.) Guill, TLCBioautography
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