Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jukut Pahit (Paspalum Conjugatum) Asal Desa Pongruan Kabupaten Manggarai Timur Terhadap Bakteri Staphylococcus Aureus Dan Kajian Senyawa Aktif
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Institusion
Universitas Katolik Widya Mandira Kupang
Author
MUSWANTO, Eufronius
Subject
L Education (General)
Datestamp
2022-11-23 00:29:18
Abstract :
The purpose of this research was to know the activity of antibacterial test of ethanol extract of jukut pahit leaves (Paspalum conjugatum) of Pongruan village of East Manggarai regency towards to the growth of Staphylococus aureus bacteria and the study of its active compund. The total of samples of jukut pahit macerated leaves were 200 g with 70% ethanol solvent, and the obtain of viscous extract is 29,90 g. The result of rendemen was 14.95%. The physical properties of the extract are: blackish brown, sticky and soluble in cold water. Phytochemical test results are: positive contains alkaloid compounds, steroids, and Saponin. The results of antibacterial activity test of Staphylococcus aureus bacteria with diffusion well method obtained by the data as follows 75% and 100% concentration have very strong inhibitory power, concentration of 1,45%, 2,25%, 5,25%, 10,25%, 12,5%, 16,25%, 25%, and 50% has a strong inhibition. Fractionation of the active compound in the sample with TLC and column chromatography using eluent ethyl acetate: chloroform (1:1 v / v) obtained 40 fractions, grouped into 6 groups of fractions based on Rf value and spot equality. Analysis of the active compound on the sample fraction using UV-Vis and infrared spectrophotometer, it was found that fraction A denotes absorption bands of ? 230 nm. Fraction B has an absorption of ? 225 nm. The C fraction has an absorption of ? 220 nm. Fraction D has an absorption of ? 225 nm. Fraction E has an absorption of ? 230 nm. The F fraction has an absorption of ? 230 nm which shows the electron transitions ???*. The infrared spectra of fractions A, B, C, D, E and F indicate the presence of an alpha, N-H, C-H alifatik, C-H aromatik, C-N, C=N, C=C dan C-O.
The purpose of this research was to know the activity of antibacterial test of ethanol extract of jukut pahit leaves (Paspalum conjugatum) of Pongruan village of East Manggarai regency towards to the growth of Staphylococus aureus bacteria and the study of its active compund. The total of samples of jukut pahit macerated leaves were 200 g with 70% ethanol solvent, and the obtain of viscous extract is 29,90 g. The result of rendemen was 14.95%. The physical properties of the extract are: blackish brown, sticky and soluble in cold water. Phytochemical test results are: positive contains alkaloid compounds, steroids, and Saponin. The results of antibacterial activity test of Staphylococcus aureus bacteria with diffusion well method obtained by the data as follows 75% and 100% concentration have very strong inhibitory power, concentration of 1,45%, 2,25%, 5,25%, 10,25%, 12,5%, 16,25%, 25%, and 50% has a strong inhibition. Fractionation of the active compound in the sample with TLC and column chromatography using eluent ethyl acetate: chloroform (1:1 v / v) obtained 40 fractions, grouped into 6 groups of fractions based on Rf value and spot equality. Analysis of the active compound on the sample fraction using UV-Vis and infrared spectrophotometer, it was found that fraction A denotes absorption bands of ? 230 nm. Fraction B has an absorption of ? 225 nm. The C fraction has an absorption of ? 220 nm. Fraction D has an absorption of ? 225 nm. Fraction E has an absorption of ? 230 nm. The F fraction has an absorption of ? 230 nm which shows the electron transitions ???*. The infrared spectra of fractions A, B, C, D, E and F indicate the presence of an alpha, N-H, C-H alifatik, C-H aromatik, C-N, C=N, C=C dan C-O.
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