Uji Aktivitas Antioksidan Dan Toksisitas Tepung Umbi Porang (Amorphophallus Oncophylllus) Asal Desa Laka’anmau Kabupaten Belu Nusa Tenggara Timur (NTT)
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Institusion
Universitas Katolik Widya Mandira Kupang
Author
TUE, Daniel
Subject
Q Science (General)
Datestamp
2022-11-23 00:33:44
Abstract :
Study of antioxidant and toxicity test of porang root flour (Amorphophallus oncophylllus), from Belu Regency, East Nusa Tenggara, it has been conducted. This research aims to determine the antioxidant capacity and toxicity effect of porang flour. Weight out about 250 grams of sample was extracted by masseration method using ethanol 70%, it was obtained 16.86 grams extract (yield is 6.75%). Furthermore, the phytochemical analysis was carried out by Harborne (1987) method, it results positif containing of alkaloid, flavonoid, dan tannin. An Antioxidant activity was determined by DPPH (2,2- Diphenyl-1 picrylhydrazil). Absorbance of sample was measured by spectrophotometer at 518 wavelength. The result of antioxidant analysis of sample obtained IC50 value amounts 408.324 ppm and it was indicated that extract of sample contained weak antioxidant value. Furthermore, toxicity was determined by BSLT (brine shrimp lethality test) method, and obtained the LC50 value amount 15.205 ppm, and it was indicated that extract of sample contained weak toxic value effect.
Study of antioxidant and toxicity test of porang root flour (Amorphophallus oncophylllus), from Belu Regency, East Nusa Tenggara, it has been conducted. This research aims to determine the antioxidant capacity and toxicity effect of porang flour. Weight out about 250 grams of sample was extracted by masseration method using ethanol 70%, it was obtained 16.86 grams extract (yield is 6.75%). Furthermore, the phytochemical analysis was carried out by Harborne (1987) method, it results positif containing of alkaloid, flavonoid, dan tannin. An Antioxidant activity was determined by DPPH (2,2- Diphenyl-1 picrylhydrazil). Absorbance of sample was measured by spectrophotometer at 518 wavelength. The result of antioxidant analysis of sample obtained IC50 value amounts 408.324 ppm and it was indicated that extract of sample contained weak antioxidant value. Furthermore, toxicity was determined by BSLT (brine shrimp lethality test) method, and obtained the LC50 value amount 15.205 ppm, and it was indicated that extract of sample contained weak toxic value effect.
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