Profil Senyawa Metabolit Sekunder dan Aktivitas Antioksidan Ekstrak n-Heksan Daun Kemangi Beberapa Jenis Kemangi yang Tumbuh di Noelbaki Kupang
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Institusion
Universitas Katolik Widya Mandira Kupang
Author
LERA, Ludgardis Osi
Subject
Q Science (General)
Datestamp
2023-02-15 23:58:48
Abstract :
Identification of secondary metabolites and antioxidant activity of Ocimum americanum, Ocimum basilicum and Ocimum tenuiflorum samples has been carried out. Basil leaf samples were extracted with n-hexane using the soxhletation method. Compound analysis was carried out using the GC-MS method and the antioxidant activity of basil leaves was analyzed using the DPPH method. The soxhletated extract was green in the Ocimum americanum and Ocimum tenuiflorum samples, while the Ocimum basilicum sample was yellow and had a distinctive smell of basil. The results of the GC-MS analysis of the three types of basil leaf samples Ocimum americanum, Ocimum tenuiflorum and Ocimum basilicum yielded 21 compounds with the main compound components namely: estragol 88.36% in the sample Ocimum americanum, 1,8-Cineol 36.56% and trans-caryophyllene 40.41% in Ocimum tenuiflorum samples, and 94.04% estragol in Ocimum basilicum samples. The test results for the antioxidant activity of the three samples of Ocimum americanum basil leaves, Ocimum tenuiflorum and Ocimum basilicum produced were classified as weak with the values of each sample Ocimum americanum 701.77 ppm, Ocimum tenuiflorum 1268.53 ppm and Ocimum basilicum 1273.55 ppm.
Identification of secondary metabolites and antioxidant activity of Ocimum americanum, Ocimum basilicum and Ocimum tenuiflorum samples has been carried out. Basil leaf samples were extracted with n-hexane using the soxhletation method. Compound analysis was carried out using the GC-MS method and the antioxidant activity of basil leaves was analyzed using the DPPH method. The soxhletated extract was green in the Ocimum americanum and Ocimum tenuiflorum samples, while the Ocimum basilicum sample was yellow and had a distinctive smell of basil. The results of the GC-MS analysis of the three types of basil leaf samples Ocimum americanum, Ocimum tenuiflorum and Ocimum basilicum yielded 21 compounds with the main compound components namely: estragol 88.36% in the sample Ocimum americanum, 1,8-Cineol 36.56% and trans-caryophyllene 40.41% in Ocimum tenuiflorum samples, and 94.04% estragol in Ocimum basilicum samples. The test results for the antioxidant activity of the three samples of Ocimum americanum basil leaves, Ocimum tenuiflorum and Ocimum basilicum produced were classified as weak with the values of each sample Ocimum americanum 701.77 ppm, Ocimum tenuiflorum 1268.53 ppm and Ocimum basilicum 1273.55 ppm.
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